Optical methods for detecting biofilms in situ

Authors

Keywords:

biofilms, optical methods, Salmonella, Listeria monocytogenes, Pseudomonas, hygiene,spectroscopy, fluorescence, in situ, cleaning and disinfection, food industry, optical fiber.

Abstract

Biofilms are highly resistant microbial structures that pose a risk to food safety, as they allow pathogenic microorganisms such as Salmonella or Listeria monocytogenes to persist on food-contact surfaces in the food industry despite standard cleaning and disinfection procedures. The presence of biofilms can cause cross-contamination, product spoilage, equipment inefficiency, and public health risks. This study reviewed available optical methods for the in situ and real-time detection of biofilms, aiming to identify techniques applicable to the food industry that enable rapid and effective detection. A classification of methods is presented based on the nature of the measurement, sampling requirements, and type of signal reading. Among the techniques reviewed, fluorescence sensors, spectroscopy, and fiber-optic-based technologies stand out as the most promising options. The combination of fixed and portable methods is also proposed as an optimal strategy for efficient biofilm monitoring and control. This approach could improve hygiene protocols and contribute to food safety through fast specific detection.

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Published

2025-12-22

How to Cite

Francisco Camprubí, N. de, Perez-Playà, B., Ripolles-Avila, C., & Castillo, M. (2025). Optical methods for detecting biofilms in situ. TECA: Tecnologia I Ciència Dels Aliments, 29–39. Retrieved from https://revistes.iec.cat/index.php/TECA/article/view/156383

Issue

Vol. 24-25 (2025)

Section

Articles

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