Estudi i diagnòstic d'aneuploïdies en oòcits humans Authors Mariona Rius Albert Obradors Maria Oliver-Bonet Jordi Benet Joaquima Navarro Abstract The availability of biologic material discarded from IVF cycles has been pretty valuable for the study of aneuploidies in the first stages of embryonic development. The cytogenetic study of human oocytes requires the analysis of the MII oocyte and the corresponding 1rstPB separately. Fluorescent in situ hybridization and its variants, such as SKY, PRINS or cenM-FISH, have been very useful, although these techniques require a previous cell fixation. As an alternative, CGH does not require cell fixation and furthermore, it allows for the study of the whole chromosomal complement. Aneuploidy rate from published CGH results show a great variability. It ranges from 22% to 65%. This variability is mainly due to the use of different material: oocytes from women undergoing IVF and fresh oocytes from donors The mechanisms proposed for aneuploidy production are mainly alterations produced in anaphase I, such as homologous non-disjunction and premature separation of sister chromatids. Mitotic segregation failure in the oogonies produced during the early stages of embryonic development has also been reported. The translational application of studies has favoured the development of PGD, by means of FISH or CGH, for the aneuploidy diagnostic of female origin. At this moment, one of the challenges is to increase the implantation rate of the transferred embryos. Downloads Text complet (Català) Published 2009-04-27 Issue 59, 2008 : Biologia de la reproducció / Mercè Durfort i Francesca Vidal, editores Section Articles License The intellectual property of the articles belongs to the respective authors. At the time of submitting the articles to Treballs de la Societat Catalana de Biologia, authors accept the following terms: — Authors assign to the SCB (a subsidiary of the Institut d’Estudis Catalans) the rights of reproduction, public communication (including communication through social networks) and distribution of the articles submitted for publication to Treballs de la Societat Catalana de Biologia, in any form and medium, including digital platforms. The Publications Committee reserves the right to accept or refuse submitted articles and the right to make any editorial changes it deems appropriate. If the suggested changes are accepted by authors, they should re-submit the article with such changes. — Authors answer to the SCB for the authorship and originality of submitted articles. In other words, authors assure that submitted articles do not contain fragments of works by other authors or fragments of their own previously published works; that the content of articles is original, and that the copyright of third parties is not infringed upon. Authors accept this responsibility and undertake to hold harmless the SCB for any loss or damage resulting from non-compliance with this obligation. Furthermore, they should include a statement in articles submitted to the journal regarding their responsibility for the content of the articles. — Authors are responsible for obtaining permission for the reproduction of all graphic material included in articles, and they should moreover ensure that images, videos, etc., have been created with the consent of the individuals appearing in them, and that material belonging to third parties is clearly identified and acknowledged as such within the text. Likewise, authors should provide the respective consents and authorisations to the SCB when submitting articles. — The SCB is exempt from any liability arising from the possible infringement of intellectual property rights by authors. In all cases, it undertakes to publish corrections, clarifications, retractions and apologies, if necessary. — Unless otherwise stated in the text or in the graphic material, the contents published in the journal are subject to an Attribution - NonCommercial - NoDerivs 3.0 Spain (by-nc-nd) license from Creative Commons, the full text of which may be consulted at https://creativecommons.org/licenses/by-nc-nd/3.0/es/deed.en. Therefore, the general public is authorised to reproduce, distribute and communicate articles as long as their authorship and publishing entity are acknowledged, and no commercial use is made of them nor derivative work produced from them. — The journal is not responsible for the ideas and opinions expressed by the authors of published articles.
