Producció in vivo versus in vitro d'embrions caprins

Authors

  • Maria Teresa Paramio Nieto
  • Dolors Izquierdo

Abstract

Assisted reproductive technologies (ART) such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been used to increase reproductive efficiency and accelerate genetic gain in male and females, respectively. The current limitations of MOET are due to: i) female variability response to hormonal treatment, ii) fertilization failures and iii) premature regression of corpora luteum. The in vitro production (IVP) of embryos offers the possibility of overcoming MOET limitations. The method of IVP of embryos involves three mains steps: in vitro maturation of oocytes (IVM), in vitro fertilization of oocytes (IVF) with capacitated sperm and in vitro culture (IVC) of embryos until blastocyst stage that can be transferred to recipient females or cryopreserved for future use. Recovering oocytes from live selected females by laparoscopic ovum pick-up (LOPU), and breeding prepubertal females by juvenile in vitro embryo technology (JIVET) will allow a high diffusion of valuable goats. Also, IVP of goat embryos will provide an excellent source of low cost.

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Published

2009-04-27