Insight into the association between phospholipase C-γ1 and the insulin receptor Authors Hyeung-Jin Jang Yong-Kook Kwon Sutapa Kole Michel Bernier Abstract An intense investigation is underway to identify the signaling intermediates that interact directly with the insulin receptor (IR) as they appear to be associated with the transmission of insulin action.We recently reported the inducible association of phospholipase C (PLC)γ1 with the IR and its involvement in the transduction of insulin-mediated signals through activation of the Ras/extracellular-regulated kinase pathway. Akin to protein phosphorylation, redox regulation represents an important metabolic modulator of cellular functions. In this study, various mutant forms of PCLγ1 were generated to identify putative key redox-sensitive cysteines that provide sites for a number of post-translational modifications. Toward this aim, two methods were used that demonstrate the presence of oxidation-sensitive cysteine residues in PCLγ1. Using site-directed mutagenesis experiments we found that two cysteines in PCLγ1 (Cys-8 and Cys-12) were modified with hydrogen peroxide and protected against a host of reactive oxygen species through pharmacological inhibition of cellular membrane oxidases. The data suggest that the amino-terminal region of PCLγ1 contains reactive cysteine-SH groups that are exquisitely sensitive to redox regulation and which are associated with efficient interaction with the IR. The importance of these findings is already asserting themselves in studies reporting the regulatory role of cellular redox in insulin signal transduction. Downloads Text complet (Català) Published 2007-12-28 Issue 56, 2005 : Endocrinologia molecular / Jaume Reventós editor Section Articles License The intellectual property of the articles belongs to the respective authors. At the time of submitting the articles to Treballs de la Societat Catalana de Biologia, authors accept the following terms: — Authors assign to the SCB (a subsidiary of the Institut d’Estudis Catalans) the rights of reproduction, public communication (including communication through social networks) and distribution of the articles submitted for publication to Treballs de la Societat Catalana de Biologia, in any form and medium, including digital platforms. The Publications Committee reserves the right to accept or refuse submitted articles and the right to make any editorial changes it deems appropriate. If the suggested changes are accepted by authors, they should re-submit the article with such changes. — Authors answer to the SCB for the authorship and originality of submitted articles. In other words, authors assure that submitted articles do not contain fragments of works by other authors or fragments of their own previously published works; that the content of articles is original, and that the copyright of third parties is not infringed upon. Authors accept this responsibility and undertake to hold harmless the SCB for any loss or damage resulting from non-compliance with this obligation. Furthermore, they should include a statement in articles submitted to the journal regarding their responsibility for the content of the articles. — Authors are responsible for obtaining permission for the reproduction of all graphic material included in articles, and they should moreover ensure that images, videos, etc., have been created with the consent of the individuals appearing in them, and that material belonging to third parties is clearly identified and acknowledged as such within the text. Likewise, authors should provide the respective consents and authorisations to the SCB when submitting articles. — The SCB is exempt from any liability arising from the possible infringement of intellectual property rights by authors. In all cases, it undertakes to publish corrections, clarifications, retractions and apologies, if necessary. — Unless otherwise stated in the text or in the graphic material, the contents published in the journal are subject to an Attribution - NonCommercial - NoDerivs 3.0 Spain (by-nc-nd) license from Creative Commons, the full text of which may be consulted at https://creativecommons.org/licenses/by-nc-nd/3.0/es/deed.en. Therefore, the general public is authorised to reproduce, distribute and communicate articles as long as their authorship and publishing entity are acknowledged, and no commercial use is made of them nor derivative work produced from them. — The journal is not responsible for the ideas and opinions expressed by the authors of published articles.
