Advances in DNA sequencing technology

Authors

  • Berta Fusté Centre Nacional d’Anàlisi Genòmica - Centre de Regulació Genòmica (CNAG-CRG), Barcelona Institute of Science and Technology (BIST)
  • Elena Vila Centre Nacional d’Anàlisi Genòmica - Centre de Regulació Genòmica (CNAG-CRG), Barcelona Institute of Science and Technology (BIST)
  • Mònica Bayés Centre Nacional d’Anàlisi Genòmica - Centre de Regulació Genòmica (CNAG-CRG), Barcelona Institute of Science and Technology (BIST)

Keywords:

genome, next-generation sequencing (NGS), sequencing by synthesis (SBS), single-molecule real-time sequencing (SMRT-seq), nanopore sequencing (ONT).

Abstract

In 1977, Frederick Sanger developed a method for determining the order of the bases of DNA fragments. This technology still works today and has been crucial in achieving such important milestones as the first complete sequencing of the human genome. The emergence of the new generation of DNA sequencing technologies (NGS) plus the great explosion of computer tools for their analysis has become a matter of routine and allows the sequencing of the genomes of microbes, plants and animals in a way that is quick, relatively cheap and highly precise. There has been a great expansion of NGS sequencing platforms over the last decade, first involving short-read and later longread NGS sequencing technologies. Although long-read NGS sequencing promised great advances in solving complex genomes, the error rates of these technologies are high. This has led to the appearance in recent years of a number of complementary sequencing methods to address the shortcomings of NGS sequencing of short and long readings.

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Issue

Vol. 72 (2022)

Section

Articles

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