Derivació de línies de cèl·lules mare embrionàries a partir de blastòmers aïllats Authors Sheyla González Nuno Costa Borges Elena Ibáñez Josep Santaló Abstract Embryonic stem cells (ESC) are usually derived from the inner cell mass (ICM) of an embryo at the blastocyst stage. However, this strategy involves the destruction of the embryoextraemand has generated a great controversy in the case of the human species. In this sense, different groups have tried to derive ESC from isolated blastomeres of embryonic stages previous to blastocyst. But there is still very few data and the frequencies of ESC derivation obtained until now are low. In this study we present a review about the status of ESC derivation from isolated blastomeres and we report the results obtained with three methods of derivation. ESC lines have been derived from 4- and 8-cell SV129 × C57Bl/6 F1 mouse blastomeres. Morula and blastocyst stages were also used, the later as a positive control. Results obtained have allowed us to determine that the most efficient method is the culture in defined medium and that there is an important influence of the embryonic stage and the number of isolated blastomeres on the derivation of ESC by the three methods applied. Downloads Text complet (Català) Published 2009-04-29 Issue 59, 2008 : Biologia de la reproducció / Mercè Durfort i Francesca Vidal, editores Section Articles License The intellectual property of the articles belongs to the respective authors. At the time of submitting the articles to Treballs de la Societat Catalana de Biologia, authors accept the following terms: — Authors assign to the SCB (a subsidiary of the Institut d’Estudis Catalans) the rights of reproduction, public communication (including communication through social networks) and distribution of the articles submitted for publication to Treballs de la Societat Catalana de Biologia, in any form and medium, including digital platforms. The Publications Committee reserves the right to accept or refuse submitted articles and the right to make any editorial changes it deems appropriate. If the suggested changes are accepted by authors, they should re-submit the article with such changes. — Authors answer to the SCB for the authorship and originality of submitted articles. In other words, authors assure that submitted articles do not contain fragments of works by other authors or fragments of their own previously published works; that the content of articles is original, and that the copyright of third parties is not infringed upon. Authors accept this responsibility and undertake to hold harmless the SCB for any loss or damage resulting from non-compliance with this obligation. Furthermore, they should include a statement in articles submitted to the journal regarding their responsibility for the content of the articles. — Authors are responsible for obtaining permission for the reproduction of all graphic material included in articles, and they should moreover ensure that images, videos, etc., have been created with the consent of the individuals appearing in them, and that material belonging to third parties is clearly identified and acknowledged as such within the text. Likewise, authors should provide the respective consents and authorisations to the SCB when submitting articles. — The SCB is exempt from any liability arising from the possible infringement of intellectual property rights by authors. In all cases, it undertakes to publish corrections, clarifications, retractions and apologies, if necessary. — Unless otherwise stated in the text or in the graphic material, the contents published in the journal are subject to an Attribution - NonCommercial - NoDerivs 3.0 Spain (by-nc-nd) license from Creative Commons, the full text of which may be consulted at https://creativecommons.org/licenses/by-nc-nd/3.0/es/deed.en. Therefore, the general public is authorised to reproduce, distribute and communicate articles as long as their authorship and publishing entity are acknowledged, and no commercial use is made of them nor derivative work produced from them. — The journal is not responsible for the ideas and opinions expressed by the authors of published articles.
