Genomics and transcriptomics characterization of genes expressed during postharvest at 4°C by the edible basidiomycete Pleurotus ostreatus Authors Lucía Ramírez Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain José Antonio Oguiza Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Gúmer Pérez Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain José Luis Lavín Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Alejandra Omarini Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Francisco Santoyo Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Manuel Alfaro Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Raúl Castanera Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Alejandra Parenti Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Elaia Muguerza Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Antonio G. Pisabarro Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spain Abstract Pleurotus ostreatus is an industrially cultivated basidiomycete with nutritional and environmental applications. Its genome, which was sequenced by the Joint Genome Institute, has become a model for lignin degradation and for fungal genomics and transcriptomics studies. The complete P. ostreatus genome contains 35 Mbp organized in 11 chromosomes, and two different haploid genomes have been individually sequenced. In this work, genomics and transcriptomics approaches were employed in the study of P. ostreatus under different physiological conditions. Specifically, we analyzed a collection ofexpressed sequence tags (EST) obtained from cut fruit bodies that had been stored at 4°C for 7 days (postharvest conditions). Studies of the 253 expressed clones that had been automatically and manually annotated provided a detailed picture of the life characteristics of the self-sustained fruit bodies. The results suggested a complex metabolism in which autophagy, RNA metabolism, and protein and carbohydrate turnover are increased. Genes involved in environment sensing and morphogenesis were expressed under these conditions. The data improve our understanding of the decay process in postharvest mushrooms and highlight the use of high-throughput techniques to construct models of living organisms subjected to different environmental conditions. Downloads PDF Published 2011-11-08 Issue Vol. 14 No. 2 (2011) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
