Repression of the acid ZrfA/ZrfB zinc-uptake system of Aspergillus fumigatus mediated by PacC under neutral, zinc-limiting conditions

Autores/as

  • Jorge Amich Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain
  • Fernando Leal Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain
  • José Antonio Calera Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Palabras clave:

Aspergillus fumigatus, zinc-uptake system, zinc, PacC

Resumen

The ZrfA and ZrfB transporters are components of a zinc uptake system of Aspergillus fumigatus, which mainly operates under acid zinc-limiting conditions. The expression of the zrfA and zrfB genes is up-regulated by the transcriptional activator ZafA in both acid and neutral, zinc-limiting media. The transcription of zafA is not influenced by PacC, which is the transcriptional regulator involved in regulating pH homeostasis in Aspergillus. However, at neutral pH the expression level of both zrfA and zrfB is significantly reduced. In this work we show that repression of these genes in neutral and alkaline zinc-limiting media is mediated by the transcriptional regulator PacC.

Biografía del autor/a

Jorge Amich, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Fernando Leal, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

José Antonio Calera, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Descargas

Publicado

2010-09-20

Número

Vol. 12 Núm. 1 (2009)

Sección

Research Articles

Licencia

Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.

If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.

Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.