Repression of the acid ZrfA/ZrfB zinc-uptake system of Aspergillus fumigatus mediated by PacC under neutral, zinc-limiting conditions

Autors/ores

  • Jorge Amich Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain
  • Fernando Leal Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain
  • José Antonio Calera Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Paraules clau:

Aspergillus fumigatus, zinc-uptake system, zinc, PacC

Resum

The ZrfA and ZrfB transporters are components of a zinc uptake system of Aspergillus fumigatus, which mainly operates under acid zinc-limiting conditions. The expression of the zrfA and zrfB genes is up-regulated by the transcriptional activator ZafA in both acid and neutral, zinc-limiting media. The transcription of zafA is not influenced by PacC, which is the transcriptional regulator involved in regulating pH homeostasis in Aspergillus. However, at neutral pH the expression level of both zrfA and zrfB is significantly reduced. In this work we show that repression of these genes in neutral and alkaline zinc-limiting media is mediated by the transcriptional regulator PacC.

Biografies de l'autor/a

Jorge Amich, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Fernando Leal, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

José Antonio Calera, Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Institute of Biochemical Microbiology, Joint Center CSIC-USAL, Department of Microbiology and Genetics, University of Salamanca , Salamanca, Spain

Descàrregues

Publicat

2010-09-20

Número

Vol. 12 Núm. 1 (2009)

Secció

Articles

Llicència

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