Identification of superficial Candida albicans germ tube antigens in a rabbit model of disseminated candidiasis. A proteomic approach

Autores/as

  • Aranzazu Sáez-Rosón Dept. of Immunology, Microbiology and Parasitology, School of Medicine and Odontology, Univ. of the Basque Country UPV/EHU, Leioa.
  • María-Jesús Sevilla Dept. of Immunology, Microbiology and Parasitology, School of Sciences and Technology, Univ. of the Basque Country UPV/EHU, Leioa.
  • María-Dolores Moragues Department of Nursing 1, Univ. of the Basque Country UPV/EHU, Leioa, Spain

Palabras clave:

Candida albicans, germ tube antibodies, invasive candidiasis, rabbit model

Resumen

The diagnosis of invasive candidiasis remains a clinical challenge. The detection by indirect immunofluorescence of Candida albicans germ-tube-specific antibodies (CAGTA), directed against germ-tube surface antigens, is a useful diagnostic tool that discriminates between colonization and invasion. However, the standardization of this technique is complicated by its reliance on subjective interpretation. In this study, the antigenic recognition pattern of CAGTA throughout experimental invasive candidiasis in a rabbit animal model was determined by means of 2D-PAGE, Western blotting, and tandem mass spectrometry (MS/MS). Seven proteins detected by CAGTA were identified as methionine synthase, inositol-3-phosphate synthase, enolase 1, alcohol dehydrogenase 1,3-phosphoglycerate kinase, 14-3-3 (Bmh1), and Egd2. To our knowledge, this is the first report of antibodies reacting with Bmh1 and Egd2 proteins in an animal model of invasive candidiasis. Although all of the antigens were recognized by CAGTA in cell-wall dithiothreitol extracts of both germ tubes and blastospores of C. albicans, immunoelectron microscopy study revealed their differential location, as the antigens were exposed on the germ-tube cell-wall surface but hidden in the inner layers of the blastospore cell wall. These findings will contribute to developing more sensitive diagnostic methods that enable the earlier detection of invasive candidiasis. [Int Microbiol 2014; 17(1):21-29]

Keywords: Candida albicans · germ tube antibodies · invasive candidiasis · rabbit model

Biografía del autor/a

Aranzazu Sáez-Rosón, Dept. of Immunology, Microbiology and Parasitology, School of Medicine and Odontology, Univ. of the Basque Country UPV/EHU, Leioa.

 

 

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Número

Vol. 17 Núm. 1 (2014)

Sección

Research Articles

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