Recovery of Pseudomonas savastanoi pv. savastanoi from symptomless shoots of naturally infected olive trees Autores/as José M. Quesada Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Amparo García Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Edson Bertolini Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain María M. López Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Ramón Penyalver Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Palabras clave: Pseudomonas savastanoi pv. savastanoi, Olea europaea L., plant epidemiology, phylloplane, phyllosphere, leaf printing Resumen Seasonal dynamics of Pseudomonas savastanoi pv. savastanoi (Psv) on stems and leaves from symptomless shoots of naturally infected olive trees was monitored in Spanish olive orchards. Data inferred from the comparison between washing of leaves and dilution-plating versus leaf printing of individual leaves suggested that Psv population sizes varied by over several orders of magnitude, among leaves sampled concurrently from the same shoot. We did not find significant differences between leaves and stems, in respect to the number of samples where Psv was isolated or detected by PCR, showing that Psv colonizes both leaves and stems. The frequencies of Psv isolation and average populations were highly variable among field plots. No correlation between Psv populations and those of non-Psv bacteria in any plant material or field plot was observed. However, where both Psv and yellow Pantoea agglomerans colonies were isolated a positive correlation was found. In a selected field plot, dynamics of Psv over three years showed significant differences between summer and the rest of seasons. The highest Psv population occurred in warm, rainy months, while low numbers were generally found in hot and dry months. [Int Microbiol 2007; 10(2):77-84] Biografía del autor/a José M. Quesada, Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Amparo García, Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Edson Bertolini, Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain María M. López, Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Ramón Penyalver, Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Center for Plant Protection and Biotechnology, Valencian Institute for Agricultural Research (IVIA), Moncada, Valencia, Spain Descargas PDF (English) Publicado 2010-01-26 Número Vol. 10 Núm. 2 (2007) Sección Research Articles Licencia Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.