UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination Autores/as Ralf Moeller German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany Thierry Douki Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Jean Cadet Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Erko Stackebrandt German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany Wayne L. Nicholson University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA Petra Rettberg German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Günther Reitz German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Gerda Horneck German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Palabras clave: Bacillus endospores, UV-radiation, spore photoproduct, germination, DNA repair Resumen The spore photoproduct (SP) is the main DNA lesion after UV-C irradiation, and its repair is crucial for the resistance of spores to UV. The aims of the present study were to assess the formation and repair of bipyrimidine photoproducts in spore DNA of various Bacillus subtilis strains using a sensitive HPLC tandem mass spectrometry assay. Strains deficient in nucleotide excision repair, spore photoproduct lyase, homologous recombination (recA), and with wild-type repair capability were investigated. Additionally, one strain deficient in the formation of major small, acid-soluble spore proteins (SASPs) was tested. In all SASP wild-type strains, UV-C irradiation generated almost exclusively SP (>95 %) but also a few by-photoproducts. In the major SASP-deficient strain, SP and by-photoproducts were generated in equal quantities. The status of the UV-induced bipyrimidine photoproducts was determined at different stages of spore germination. After a germination time of 60 min, >75% of the SP was repaired in wild-type strains and in the SASP-deficient strain, while half of the photoinduced SP was removed in the recA-deficient strain. SP-lyase-deficient spores repaired < 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination. [Int Microbiol 2007; 10(1):39-46] Biografía del autor/a Ralf Moeller, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany Thierry Douki, Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Jean Cadet, Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France Erko Stackebrandt, German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany Wayne L. Nicholson, University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA Petra Rettberg, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Günther Reitz, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Gerda Horneck, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany Descargas PDF (English) Publicado 2010-01-27 Número Vol. 10 Núm. 1 (2007) Sección Research Articles Licencia Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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