Identification and partial purification of K88ab Escherichia coli receptor proteins in porcine brush border membranes

Autores/as

  • M. Caloca Departamento de Bioquímica y Biología Molecular, Universidad de León, León, Spain
  • S. Suárez Departamento de Sanidad Animal, Universidad de León, León, Spain
  • J. Soler Departamento de Bioquímica y Biología Molecular, Universidad de León, León, Spain

Palabras clave:

K88ab fimbriae, receptor proteins, brush border membranes

Resumen

Six receptor proteins, with molecular masses ranging from 94 to 27 kDa, that bind to Escherichia coli K88ab fimbriae were recovered from brush border membranes and were detected after solubilization with Triton X-114. The recovery of these receptor proteins in the aqueous phase suggests their peripheral localization. The 63-, 60- and 33-kDa K88ab binding proteins were recovered using gel-filtration chromatography of the aqueous phase.

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Publicado

2010-03-10

Número

Vol. 5 Núm. 2 (2002)

Sección

Research Articles

Licencia

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