Yarrowia lipolytica: a model organism for protein secretion studies

Autores/as

  • Jean-Marie Beckerich Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, Institut National Agronomique, Thiverval-Grignon, France
  • Anita Boisramé-Baudevin Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, Institut National Agronomique, Thiverval-Grignon, France
  • Claude Gaillardin Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, Institut National Agronomique, Thiverval-Grignon, France

Palabras clave:

Yarrowia lipolytica, yeast physiology, translocation, endoplasmic

Resumen

This paper reviews the advantages of the yeast Yarrowia lipolytica as a tool in the study of protein secretion. Work has been focused on the early steps leading the polypeptide, from the cytoplasmic ribosomes where it is synthesized, to the lumen of the endoplasmic reticulum. Using a thermosensitive allele of the 7SL RNA, the first in vivo evidence for a co-translational translocation was shown. Genetic screens allowed the identification of several new components of the translocation apparatus: Sls1p, an ER lumenal component involved in both translocation and lumenal transit; Tsr1p, involved in SRP-ribosome targeting; Tsr3p. Major translocation partners were also identified by reverse genetics (Sec61p, Sec62p, Kar2p, Srp54p, Sec65p).

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Publicado

2010-03-18

Número

Vol. 1 Núm. 1 (1998)

Sección

Research Articles

Licencia

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