Phenotypic comparison of clinical and plant-beneficial strains of Pantoea agglomerans

Autores/as

  • Anna Bonaterra Institute of Food and Agricultural Technology, University of Girona, Girona.
  • Esther Badosa Institute of Food and Agricultural Technology, University of Girona, Girona.
  • Fabio Rezzonico Agroscope Changins-Wädenswil ACW, Division of Plant Protection, Wädenswil, Switzerland; Zurich University of Applied Sciences ZHAW, LSFM-IUNR, Environmental Genomics and Systems Biology Research Group, Wädenswil, Switzerland
  • Brion Duffy Agroscope Changins-Wädenswil ACW, Division of Plant Protection, Wädenswil, Switzerland; Zurich University of Applied Sciences ZHAW, LSFM-IUNR, Environmental Genomics and Systems Biology Research Group, Wädenswil, Switzerland
  • Emilio Montesinos Institute of Food and Agricultural Technology, University of Girona, Girona

Palabras clave:

Pantoea agglomerans, Erwinia amylovora, Meloidogyne javanica, Penicillium expansum, Caenorhabditis elegans, biocontrol, biosafety, toxicity, hemolytic activity, Ames test

Resumen

Certain strains of Pantoea are used as biocontrol agents for the suppression of plant diseases. However, their commercial registration is hampered in some countries because of biosafety concerns. This study compares clinical and plant-beneficial strains of P. agglomerans and related species using a phenotypic analysis approach in which plant-beneficial effects, adverse effects in nematode models, and toxicity were evaluated. Plant-beneficial effects were determined as the inhibition of apple fruit infection by Penicillium expansum and apple flower infection by Erwinia amylovora. Clinical strains had no general inhibitory activity against infection by the fungal or bacterial plant pathogens, as only one clinical strain inhibited P. expansum and three inhibited E. amylovora. By contrast, all biocontrol strains showed activity against at least one of the phytopathogens, and three strains were active against both. The adverse effects in animals were evaluated in the plant-parasitic nematode Meloidogyne javanica and the bacterial-feeding nematode Caenorhabditis elegans. Both models indicated adverse effects of the two clinical strains but not of any of the plant-beneficial strains. Toxicity was evaluated by means of hemolytic activity in blood, and genotoxicity with the Ames test. None of the strains, whether clinical or plant-beneficial, showed any evidence of toxicity. [Int Microbiol 2014; 17(2):81-90]

Keywords: Pantoea agglomerans · Erwinia amylovora · Meloidogyne javanica · Penicillium expansum · Caenorhabditis elegans · biocontrol · biosafety · toxicity · hemolytic activity · Ames test

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Número

Vol. 17 Núm. 2 (2014)

Sección

Research Articles

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