A new set of DNA macrochips for the yeast Saccharomyces cerevisiae: features and uses Authors Trinidad M. Alberola DNA Chips Service-SCSIE and Department of Biochemistry and Molecular Biology, University of Valencia, Spain José García-Martínez DNA Chips Service-SCSIE and Department of Biochemistry and Molecular Biology, University of Valencia, Spain Oreto Antúnez DNA Chips Service-SCSIE and Department of Biochemistry and Molecular Biology, University of Valencia, Spain Laia Viladevall DNA Microchips and Sequencing Service and Department of Biochemistry and Molecular Biology, Autonomous University of Barcelona, Bellaterra, Spain Anna Barceló DNA Microchips and Sequencing Service and Department of Biochemistry and Molecular Biology, Autonomous University of Barcelona, Bellaterra, Spain Joaquín Ariño DNA Microchips and Sequencing Service and Department of Biochemistry and Molecular Biology, Autonomous University of Barcelona, Bellaterra, Spain José E. Pérez-Ortín DNA Chips Service-SCSIE and Department of Biochemistry and Molecular Biology, University of Valencia, Spain Keywords: Saccharomyces cerevisiae, DNA chip, macroarray Abstract The yeast Saccharomyces cerevisiae has been widely used for the implementation of DNA chip technologies. For this reason and due to the extensive use of this organism for basic and applied studies, yeast DNA chips are being used by many laboratories for expression or genomic analyses. While membrane arrays (macroarrays) offer several advantages, for many laboratories they are not affordable. Here we report that a cluster of four Spanish molecular-biology yeast laboratories, with relatively small budgets, have developed a complete set of probes for the genome of S. cerevisiae. These have been used to produce a new type of macroarray on a nylon surface. The macroarrays have been evaluated and protocols for their use have been optimized. [Int Microbiol 2004; 7(3):199-206] Downloads PDF Published 2010-02-28 Issue Vol. 7 No. 3 (2004) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
