Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain

Authors

  • Paola Caruso Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di Ricerca Olivicoltuta, Frutticoltura e Agrumicoltura (CREA)
  • Elena G. Biosca Departamento de Microbiología y Ecología, Universitat de València
  • Edson Bertolini Departamento de Fitossanidade, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS)
  • Ester Marco-Noales Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA)
  • María Teresa Gorris Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA)
  • Concetta Licciardello Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di Ricerca Olivicoltuta, Frutticoltura e Agrumicoltura (CREA)
  • María M. López Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA)

Keywords:

Bacterial wilt, potato, soil, PFGE, AFLP

Abstract

The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were performed.
The biochemical and metabolic characterization, serological tests, rep-PCR typing and phylogenetic analysis showed that all analysed strains belonged to phylotype II sequevar 1 and shared homogeneous profiles. However, interesting differences among strains were found by AFLP and macrorestriction with XbaI followed by PFGE techniques, some profiles being related to the geographical origin of the strains. Diversity results obtained offer new insights into the biogeography of this quarantine organism and its possible sources and reservoirs in Spain and Mediterranean countries.

Keywords: Bacterial wilt · potato · soil · PFGE · AFLP

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Published

2018-03-26

Issue

Vol. 20 No. 4 (2017)

Section

Research Articles

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