IS200 and multilocus sequence typing for the identification of Salmonella enterica serovar Typhi strains from Indonesia Authors Areli Martínez-Gamboa National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City. Claudia Silva Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos. Marcos Fernández-Mora Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos. Magdalena Wiesner Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos. Alfredo Ponce de León National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City. Edmundo Calva Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos. Keywords: Salmonella Typhi, bacterial molecular typing, multilocus sequence typing (MLST), clonal complex, insertion sequence IS200 Abstract In this work, IS200 and multi-locus sequence typing (MLST) were used to analyze 19 strains previously serotyped as Salmonella enterica serovar Typhi and isolated in Indonesia (16 strains), Mexico (2 strains), and Switzerland (1 strain). Most of the strains showed the most common Typhi sequence types, ST1 and ST2, and a new Typhi genotype (ST1856) was described. However, one isolate from Mexico and another from Indonesia were of the ST365 and ST426 sequence types, indicating that they belonged to serovars Weltevreden and Aberdeen, respectively. These results were supported by the amplification of IS200 fragments, which rapidly distinguish Typhi from other serovars. Our results demonstrate the utility of IS200 and MLST in the classification of Salmonella strains into serovars. These methods provide information on the clonal relatedness of strains isolated worldwide. [Int Microbiol 2015; 18(2):99-104]Keywords: Salmonella Typhi · bacterial molecular typing · multilocus sequence typing (MLST) · clonal complex · insertion sequence IS200 Author Biography Claudia Silva, Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos. Institute of Biotechnology,Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos, Mexico Downloads PDF Issue Vol. 18 No. 2 (2015) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
