Bacterial adhesion efficiency on implant abutments: A comparative study

Autors/ores

  • Marina Etxeberria Laboratory of Molecular Microbiology and Antimicrobials. Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona.
  • Lidia López-Jiménez Laboratory of Molecular Microbiology and Antimicrobials. Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona
  • Alexandra Merlos Laboratory of Molecular Microbiology and Antimicrobials. Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona.
  • Tomás Escuín Laboratory of Prosthodontics, Department of Dentistry, Medical and Dentistry Schools, University of Barcelona, IDIBELL, Barcelona, Spain
  • Miguel Viñas 1Laboratory of Molecular Microbiology and Antimicrobials. Department of Pathology and Experimental Therapeutics, University of Barcelona, Barcelona, Spain.

Paraules clau:

implant abutments, glass fiber, bacterial adhesion, nano-roughness, wettability, biomaterials

Resum

The attachment of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 28213 onto six different materials used to manufacture dental implant abutments was quantitatively determined after 2 and 24 h of contact between the materials and the bacterial cultures. The materials were topographically characterized and their wettability determined, with both parameters subsequently related to bacterial adhesion. Atomic force microscopy, interferometry, and contact angle measurement were used to characterize the materials’ surfaces. The results showed that neither roughness nor nano-roughness greatly influenced bacterial attachment whereas wettability strongly correlated with adhesion. After 2 h the degree of E. coli attachment markedly differed depending on the material whereas similar differences were not observed for S. aureus, which yielded consistently higher counts of adhered cells. Nevertheless, after 24 h the adhesion of the two species to the different test materials no longer significantly differed, although on all surfaces the numbers of finally adhered E. coli were higher than those of S. aureus. [Int Microbiol 2013; 16(4):235-242]

Keywords: implant abutments · glass fiber · bacterial adhesion · nano-roughness · wettability · biomaterials

Descàrregues

Número

Vol. 16 Núm. 4 (2013)

Secció

Articles

Llicència

Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.

If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.

Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.