Destruction of single species biofi lms of Escherichia coli or Klebsiella pneumoniae subsp. pneumoniae by dextranase, lactoferrin, and lysozyme

Autors/ores

  • Cynthia L. Sheffield
  • Tawni L. Crippen
  • Toni L. Poole
  • Ross C. Beier

Resum

The aim of this work was to determine the destructive activity of dextranase, lactoferrin, and lysozyme, against single species biofi lms composed of either Klebsiella pneumoniae subsp. pneumoniae or Escherichia coli using the MBEC Assay. Luminescence measurements based on quantitation of the ATP present were used to determine the amount of biofi lm elimination and correlated with quantity of live bacteria present in the sample. The data were analyzed employing a two-way ANOVA and Bonferroni post-test. Treatments resulted in percentage reductions of E. coli biofi lms ranging from 73 to 98 %. Lactoferrin (40 μg/ml) produced a signifi cantly higher-percentage reduction than lysozyme (10 μg/ml) (P < 0.05), no other signifi cant differences occurred. Similar treatments resulted in percentage reductions of K. pneumoniae subsp. pneumoniae biofilms ranging from 51 to 100 %. Dextranase treatments produced a signifi cantly lower percentage reduction than all other materials (P < 0.05), no other signifi cant differences occurred. No material was capable of complete destruction of both single species biofi lms; however, low concentrations of lactoferrin and lysozyme each removed 100 % of the K. pneumoniae subsp. pneumoniae biofi lm. Low concentrations of lactoferrin or lysozyme might be benefi cial to prevent biofi lm formation by K. pneumoniae subsp. pneumoniae. [Int Microbiol 2012; 15(4):183-187]

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Número

Vol. 15 Núm. 4 (2012)

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Articles

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