A protocol for PCR in situ hybridization of hyphomycetes

Autors/ores

  • Katja Sterflinger Geomicrobiology, ICBM, Carl von Ossietzky Universität, Oldenburg, Germany
  • Wolfgang E. Krumbein Geomicrobiology, ICBM, Carl von Ossietzky Universität, Oldenburg, Germany
  • Andreas Schwiertz Deutsches Institut für Ernährungsforschung, Bergholz-Rehbruecke, Germany

Paraules clau:

hyphomycetes, in situ hybridization, In situ PCR, rock inhabiting fungi, fungal ecology

Resum

A protocol for application of Polymerase Chain Reaction (PCR) in situ hybridization for the detection of hyphomycetes is presented. The experiments are exemplary carried out with strains of the genera Penicillium and Cladosporium. The small ribosomal subunit is amplified in situ by PCR using fungal specific primers. The amplicon is used as target region for a fluorescein-marked probe. The permeability of the fungal cell wall for the primers and the probe can be successfully achieved by enzymatic treatment with ß-glucanase. The protocol can be used as a basis for further development of in situ hybridization with taxon specific probes

Descàrregues

Publicat

2010-03-17

Número

Vol. 1 Núm. 3 (1998)

Secció

Articles

Llicència

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