Archaeal and bacterial diversity in two hot springs from geothermal regions in Bulgaria as demostrated by 16S rRNA and GH-57 genes

Autors/ores

  • Katerina Stefanova AgroBioInstitute, Sofia, Bulgaria
  • Iva Tomova Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Anna Tomova Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Nadja Radchenkova Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Ivan Atanassov AgroBioInstitute, Sofia, Bulgaria.
  • Margarita Kambourova Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

Paraules clau:

Archaea, hot spring, phylogenetic analysis, 16S rRNA gene, GH-57 gene

Resum

Archaeal and bacterial diversity in two Bulgarian hot springs, geographically separated with different tectonic origin and different temperature of water was investigated exploring two genes, 16S rRNA and GH-57. Archaeal diversity was significantly higher in the hotter spring Levunovo (LV) (82°C); on the contrary, bacterial diversity was higher in the spring Vetren Dol (VD) (68°C). The analyzed clones from LV library were referred to twenty eight different sequence types belonging to five archaeal groups from Crenarchaeota and Euryarchaeota. A domination of two groups was observed, Candidate Thaumarchaeota and Methanosarcinales. The majority of the clones from VD were referred to HWCG (Hot Water Crenarchaeotic Group). The formation of a group of thermophiles in the order Methanosarcinales was suggested. Phylogenetic analysis revealed high numbers of novel sequences, more than one third of archaeal and half of the bacterial phylotypes displayed similarity lower than 97% with known ones. The retrieved GH-57 gene sequences showed a complex phylogenic distribution. The main part of the retrieved homologous GH-57 sequences affiliated with bacterial phyla Bacteroidetes, Deltaproteobacteria, Candidate Saccharibacteria and affiliation of almost half of the analyzed sequences is not fully resolved. GH-57 gene analysis allows an increased resolution of the biodiversity assessment and in depth analysis of specific taxonomic groups. [Int Microbiol 18(4):217-223 (2015)]

Keywords: Archaea · hot spring · phylogenetic analysis · 16S rRNA gene · GH-57 gene

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Número

Vol. 18 Núm. 4 (2015)

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Articles

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