Antagonism of entomopathogenic fungi by Bacillus spp. associated with the integument of cicadellids and delphacids Autors/ores Andrea Toledo Plant Pathology Research Center, Faculty of Agricultural Sciences and Forestry, National University of La Plata, La Plata, Buenos Aires. Silvina López Institute of Plant Physiology, Faculty of Agricultural Sciences and Forestry, National University of La Plata- CONICET, La Plata, Buenos Aires. Mónica Aulicino Institute of Phytotechnology Santa Catalina, Faculty of Agricultural Sciences and Forestry, National University of La Plata, Lavallol, Buenos Aires. Ana María de Remes Lenicov Entomology Division, Faculty of Natural Sciences and Museum, National University of La Plata, La Plata, Buenos Aires. Pedro Balatti Plant Pathology Research Center, Faculty of Agricultural Sciences and Forestry, National University of La Plata, La Plata, Buenos Aires. Paraules clau: Bacillus spp., antagonism, entomopathogenic fungi, Cicadellidae, Delphacidae Resum Entomopathogenic fungi are potential tools to biocontrol cicadellids and delphacids, two groups of insects that cause extensive damage to agricultural crops. However, bacteria living on the host cuticle may inhibit fungal growth. In the present work, following the molecular characterization of 10 strains of Bacillus isolated from the integument of cicadellids and delphacids, we selected isolates of the fungi Beauveria bassiana and Metarhizium anisopliae that are resistant to the antimicrobials secreted by these bacterial strains. The antagonistic activity of the 10 bacterial isolates belonging to the genus Bacillus (i.e., B. amyloliquefaciens, B. pumilus, and B. subtilis) against 41 isolates of Bea. bassiana and 20 isolates of M. anisopliae was investigated in vitro on tryptic soy agar using the central disk test. With this approach, isolates of Bea. bassiana and M. anisopliae resistant to antagonistic bacteria were identified that can be further developed as biological control agents. [Int Microbiol 2015; 18(2):91-97]Keywords: Bacillus spp. · antagonism · entomopathogenic fungi · Cicadellidae · Delphacidae Biografia de l'autor/a Mónica Aulicino, Institute of Phytotechnology Santa Catalina, Faculty of Agricultural Sciences and Forestry, National University of La Plata, Lavallol, Buenos Aires. Descàrregues PDF (English) Número Vol. 18 Núm. 2 (2015) Secció Articles Llicència Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.