A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil

Autors/ores

  • Itácio Q.M. Padilha Department of Biotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa
  • Susana V. Valenzuela Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona
  • Teresa C.S.L. Grisi Department of Biotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa
  • Pilar Diaz Department of Microbiology, Faculty of Biology. University of Barcelona, Barcelona
  • Demetrius A.M. de Araújo Department of Biotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa
  • Francisco I. Javier Pastor FIJ. Pastor. University of Barcelona

Paraules clau:

Paenibacillus favisporus, xylanase, glycosyl hydrolases GH30

Resum

A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg–1, a Km of 4.0 mg/ml, and a kcat of 13,333 min–1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184]

Keywords: Paenibacillus favisporus · xylanase · glycosyl hydrolases GH30

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