Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi

Autors/ores

  • Rosalia Trias Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona
  • Lluís Bañeras Molecular Microbial Ecology Group, IEA, University of Girona
  • Emilio Montesinos Molecular Microbial Ecology Group, IEA, University of Girona
  • Esther Badosa Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona

Paraules clau:

Penicillium expansum, lactic acid bacteria (LAB), biocontrol, spoilage, fresh fruit

Resum

The aim of the study was to evaluate the efficacy of lactic acid bacteria isolated from fresh fruits and vegetables as biocontrol agents against the phytopathogenic and spoilage bacteria and fungi, Xanthomonas campestris, Erwinia carotovora, Penicillium expansum, Monilinia laxa, and Botrytis cinerea. The antagonistic activity of 496 lactic acid bacteria strains was tested in vitro, and all tested microorganisms except P. expansum were inhibited by at least one isolate. The 496 isolates were also tested for inhibition of infection of P. expansum in wounds of Golden Delicious apples. Four strains TC97, AC318, TM319 and FF441 reduced the fungal rot diameter of apples by 20%, and only, Weissella cibaria strain TM128 decreased infection levels by 50%. Cell-free supernatants of selected antagonistic bacteria were studied to determine the nature of the antimicrobial compounds produced. Organic acids were the preferred inhibition mechanism but hydrogen peroxide was also detected when strains BC48, TM128, PM141 and FF441 were tested against Erwinia carotovora. These results support the potentiality of lactic acid bacteria as biocontrol agents against postharvest rot. Previous works reporting of antifungal activity by lactic acid bacteria are scarce.

Biografies de l'autor/a

Rosalia Trias, Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona

Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona

Lluís Bañeras, Molecular Microbial Ecology Group, IEA, University of Girona

Molecular Microbial Ecology Group, IEA, University of Girona

Emilio Montesinos, Molecular Microbial Ecology Group, IEA, University of Girona

Molecular Microbial Ecology Group, IEA, University of Girona

Esther Badosa, Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona

Institute for Food and Agricultural Technology-CeRTA, CIDSAV, University of Girona

Descàrregues

Publicat

2010-09-21

Número

Vol. 11 Núm. 4 (2008)

Secció

Articles

Llicència

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