Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a

Authors

  • Gonzalo Platas Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Spain
  • Inmaculada Meseguer División de Microbiología, Campus de Elche, Universidad Miguel Hernández, Elche, Alicante, Spain
  • Ricardo Amils Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain

Keywords:

halocin, bacteriocin, Haloferax mediterranei, Haloarchaea, halophiles

Abstract

The production of halocins, bacteriocin-like proteins of ecological significance, is a frequent characteristic of species from the family Halobacteriaceae. Halocin H1, produced by Haloferax mediterranei strain M2a, is a single 31-kDa polypeptide. Its purification was achieved by combining two chromatographic systems: Sepharose 4B linked to bacitracin followed by hydroxylapatite Bio-gel HTP. Halocin H1 required concentrations of NaCl higher than 1.5 M to maintain its activity. Haoarchaeal strains showed a differential degree of sensitivity to the action of this halocin.

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Published

2010-03-10

Issue

Vol. 5 No. 1 (2002)

Section

Research Articles

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