Identification of Vibrio spp. (other than V. vulnificus) recovered on CPC agar from marine natural samples

Authors

  • María Carmen Macián Department of Microbiology, Faculty of Biology, University of Valencia, Burjassot, Spain
  • Covadonga R. Arias Department of Microbiology, Faculty of Biology, University of Valencia, Burjassot, Spain
  • Rosa Aznar Department of Microbiology, Faculty of Biology, University of Valencia, Burjassot, Spain; and Institute of Agrochemistry and Food Technology, CSIC, Burjassot, Valencia, Spain
  • Esperanza Garay Department of Microbiology, Faculty of Biology, University of Valencia, Burjassot, Spain; and Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Burjassot, Spain
  • María Jesús Pujalte Department of Microbiology, Faculty of Biology, University of Valencia, Burjassot, Spain; and Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, Burjassot, Spain

Keywords:

Vibrio spp., Vibrio vulnificus, marine bacteria, phenotypic characterization, CPC agar

Abstract

Two hundred and eighty four presumptive but not confirmed Vibrio vulnificus isolates grown on cellobiose-polymixin B-colistin agar (CPC) at 40°C, recovered from sea water samples from Valencia, Spain, during a microbiological survey for V. vulnificus, were phenotypically identified. Most of the isolates (91%) corresponded to Vibrio species. V. harveyi (24%) and V. splendidus (19%) were the most abundant species identified, followed by V. navarrensis (13%), V. alginolyticus (8%) and V. parahaemolyticus (5%). The ability to grow on CPC agar and ferment cellobiose of several V. vulnificus strains from different origins and serovars, including reference strains, was tested. Most serovar E isolates and 25% of non-serovar E isolates could not grow on CPC agar.

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Published

2010-03-15

Issue

Section

Research Articles