Rapid identification of Salmonella typhimurium, S. enteritidis and S. virchow isolates by Polymerase Chain Reaction based fingerprinting methods Authors Antonio Bennasar Department of Biology, Microbiology, University of the Balearic Islands, and Mediterranean Institute of Advanced Studies (CSIC–UIB), Spain; and Bereich Mikrobiologie, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany Gloria de Luna Ministry of Health and Social Security, Autonomous Government of the Balearic Islands, Palma de Mallorca, Spain Bartomeu Cabrer Ministry of Health and Social Security, Autonomous Government of the Balearic Islands, Palma de Mallorca, Spain Jorge Lalucat Department of Biology, Microbiology, University of the Balearic Islands, and Mediterranean Institute of Advanced Studies (CSIC–UIB), Spain Keywords: Salmonella, 16S–23S rDNA spacer region, enterobacterial repetitive intergenic consensus (ERIC), molecular typing, repetitive extragenic palindromic (REP) sequences Abstract In this study we used and evaluated three rapid molecular typing methods for the identification of three frequent, clinically significant Salmonella serovars on the basis of the ease, simplicity and reproducibility of the chosen methods. We determined the genetic diversity among several isolates of Salmonella enteritidis, S. typhimurium and S. virchow, and compared them with other enterobacteria by using the repetitive extragenic palindromic (REP) sequences, the enterobacterial repetitive intergenic consensus (ERIC) sequences, and the 16S–23S rDNA intergenic spacer region (ITS1). The objective was to evaluate their potential application to discriminate among members of the species Salmonella enterica subspecies enterica using the genetic diversity of the group found by genomic fingerprinting. The three different serovars of Salmonella studied gave reproducible and distinguishable profiles using whichever of the above mentioned polymerase chain reaction (PCR) methods assayed. The conserved patterns in each serovar allowed for easy differentiation from other serovars of Salmonella. Downloads PDF Published 2010-03-15 Issue Vol. 3 No. 1 (2000) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
