UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination

Authors

  • Ralf Moeller German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany
  • Thierry Douki Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France
  • Jean Cadet Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France
  • Erko Stackebrandt German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany
  • Wayne L. Nicholson University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA
  • Petra Rettberg German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
  • Günther Reitz German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
  • Gerda Horneck German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

Keywords:

Bacillus endospores, UV-radiation, spore photoproduct, germination, DNA repair

Abstract

The spore photoproduct (SP) is the main DNA lesion after UV-C irradiation, and its repair is crucial for the resistance of spores to UV. The aims of the present study were to assess the formation and repair of bipyrimidine photoproducts in spore DNA of various Bacillus subtilis strains using a sensitive HPLC tandem mass spectrometry assay. Strains deficient in nucleotide excision repair, spore photoproduct lyase, homologous recombination (recA), and with wild-type repair capability were investigated. Additionally, one strain deficient in the formation of major small, acid-soluble spore proteins (SASPs) was tested. In all SASP wild-type strains, UV-C irradiation generated almost exclusively SP (>95 %) but also a few by-photoproducts. In the major SASP-deficient strain, SP and by-photoproducts were generated in equal quantities. The status of the UV-induced bipyrimidine photoproducts was determined at different stages of spore germination. After a germination time of 60 min, >75% of the SP was repaired in wild-type strains and in the SASP-deficient strain, while half of the photoinduced SP was removed in the recA-deficient strain. SP-lyase-deficient spores repaired < 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination. [Int Microbiol 2007; 10(1):39-46]

Author Biographies

Ralf Moeller, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

Thierry Douki, Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France

Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France

Jean Cadet, Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France

Service of Inorganic and Biological Chemistry, UMR-E3 (CEA/UJF), Department of Fundamental Research on Condensed Matter, CEA-Grenoble, France

Erko Stackebrandt, German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

Wayne L. Nicholson, University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA

University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA

Petra Rettberg, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

Günther Reitz, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

Gerda Horneck, German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany

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Published

2010-01-27

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Section

Research Articles