HeLa-cell adherence patterns and actin aggregation of enteropathogenic Escherichia coli (EPEC) and Shiga-toxin-producing E. coli (STEC) strains carrying different eae and tir alleles Authors Azucena Mora Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Miguel Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Denise Yamamoto Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Ghizlane Dahbi Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Jesús E. Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Cecilia López Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain María P. Alonso Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain Mônica A. M. Vieira Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Rodrigo T. Hernandes Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Cecilia M. Abe Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil Roxane M. F. Piazza Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil David W. Lacher Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA Waldir P. Laboratory of Bacteriology, Butantan Ins Elias Tania A. T. Gomes Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Jorge Blanco Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Keywords: enteropathogenic E. coli, Shiga-toxin-producing E. coli, HeLa-cell adherence, intimin Abstract A collection of 69 eae-positive strains expressing 29 different intimin types and eight tir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BFP) expression. All of the nine typical enteropathogenic Escherichia coli (tEPEC) studied harbored the enteropathogenic E. coli adherence factor (EAF) plasmid, as shown by PCR and/or EAF probe results. In addition, they were positive for bfpA, as shown by PCR, and BFP expression, as confirmed by immunofluorescence (IFL) and/or immunoblotting (IBL) assays. Localized adherence (LA) was exclusively displayed by those nine tEPEC, while localized-adherence-like (LAL) was the most frequent pattern among atypical EPEC (aEPEC) and Shiga-toxinproducing E. coli (STEC). All LA and LAL strains were able to cause attaching and effacing (AE) lesions, as established by means of the FAS test. There was a significant association between the presence of tir allele α1 and bfpA-positive strains, and consequently, with the LA pattern. However, intimin type or bfpA was not associated with the adherence pattern displayed in HeLa cells. Among the eight bfpA alleles detected, a new type (β10; accession number FN391178) was identified in a strain of serotype O157:H45, and a truncated variant (β3.2-t; accession number FN 391181) in four strains belonging to different pathotypes. [Int Microbiol 2009; 12(4):243-251] Author Biographies Azucena Mora, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Miguel Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Denise Yamamoto, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Ghizlane Dahbi, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Jesús E. Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Cecilia López, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain María P. Alonso, Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain Unit of Microbiology, Calde Xeral Hospital, Lugo, Spain Mônica A. M. Vieira, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Rodrigo T. Hernandes, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Cecilia M. Abe, Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil Roxane M. F. Piazza, Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil David W. Lacher, Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA Division of Molecular Biology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, USA Waldir P. Laboratory of Bacteriology, Butantan Ins Elias Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil Tania A. T. Gomes, Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Department of Microbiology, Immunology and Parasitology, São Paulo School of Medicine, Federal University of São Paulo, Brazil Jorge Blanco, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain Downloads PDF Published 2010-01-21 Issue Vol. 12 No. 4 (2009) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
