NAD+-dependent post-translational modification of Escherichia coli glyceraldehyde-3-phosphate dehydrogenase Authors Laura Aguilera Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Rosa Giménez Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Josefa Badia Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Juan Aguilar Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Laura Baldoma Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Keywords: Escherichia coli, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), secreted proteins, ADP-ribosylation, covalent modification of proteins Abstract Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional housekeeping protein reported to be a target of several covalent modifications in many organisms. In a previous study, enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains were shown to secrete GAPDH and the protein to bind human plasminogen and fibrinogen. Here we report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site. ADP-ribosylation of extracellular GAPDH may play an important role in the host-pathogen interaction, as also proposed in other pathogens. [Int Microbiol 2009; 12(3):187-192] Author Biographies Laura Aguilera, Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Rosa Giménez, Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Josefa Badia, Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Juan Aguilar, Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Laura Baldoma, Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Department of Biochemistry and Molecular Biology, Institute of Biomedicine of the University of Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona, Barcelona, Spain Downloads PDF Published 2010-01-14 Issue Vol. 12 No. 3 (2009) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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