Assessment of human enteric viruses in cultured and wild bivalve molluscs

Authors

  • M. Luz Vilariño Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain
  • Françoise S. Le Guyader Laboratory of Microbiology, IFREMER, Nantes, France
  • David Polo Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain
  • Julien Schaeffer Laboratory of Microbiology, IFREMER, Nantes, France
  • Joanna Kröl Laboratory of Microbiology, IFREMER, Nantes, France
  • Jesús L. Romalde Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Keywords:

molluscs, enteric viruses, hepatitis A virus, norovirus, viral prevalence, viral quantification, seafood industry

Abstract

Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ría de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis Avirus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 × 102 to 3.3 × 103 RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 × 101 to 1.5 × 104 RNA copies per gram of digestive tissue) than in wild (from 1.3 × 102 to 3.4 × 104 RNA copies per gram of digestive tissue) samples. [Int Microbiol 2009; 12(3):145-151]

Author Biographies

M. Luz Vilariño, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Françoise S. Le Guyader, Laboratory of Microbiology, IFREMER, Nantes, France

Laboratory of Microbiology, IFREMER, Nantes, France

David Polo, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Julien Schaeffer, Laboratory of Microbiology, IFREMER, Nantes, France

Laboratory of Microbiology, IFREMER, Nantes, France

Joanna Kröl, Laboratory of Microbiology, IFREMER, Nantes, France

Laboratory of Microbiology, IFREMER, Nantes, France

Jesús L. Romalde, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

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Published

2010-01-14

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Section

Research Articles