Assessment of human enteric viruses in cultured and wild bivalve molluscs Authors M. Luz Vilariño Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Françoise S. Le Guyader Laboratory of Microbiology, IFREMER, Nantes, France David Polo Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Julien Schaeffer Laboratory of Microbiology, IFREMER, Nantes, France Joanna Kröl Laboratory of Microbiology, IFREMER, Nantes, France Jesús L. Romalde Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Keywords: molluscs, enteric viruses, hepatitis A virus, norovirus, viral prevalence, viral quantification, seafood industry Abstract Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ría de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis Avirus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 × 102 to 3.3 × 103 RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 × 101 to 1.5 × 104 RNA copies per gram of digestive tissue) than in wild (from 1.3 × 102 to 3.4 × 104 RNA copies per gram of digestive tissue) samples. [Int Microbiol 2009; 12(3):145-151] Author Biographies M. Luz Vilariño, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Françoise S. Le Guyader, Laboratory of Microbiology, IFREMER, Nantes, France Laboratory of Microbiology, IFREMER, Nantes, France David Polo, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Julien Schaeffer, Laboratory of Microbiology, IFREMER, Nantes, France Laboratory of Microbiology, IFREMER, Nantes, France Joanna Kröl, Laboratory of Microbiology, IFREMER, Nantes, France Laboratory of Microbiology, IFREMER, Nantes, France Jesús L. Romalde, Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain Downloads PDF Published 2010-01-14 Issue Vol. 12 No. 3 (2009) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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