Inhibitory activity of Phaeobacter strains against aquaculture pathogenic bacteria Authors Susana Prado Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Jaime Montes Center of Marine Research, Ministry of Fisheries and Marine Affairs, Autonomous Government of Galicia, Vilanova de Arousa, Spain Jesús L. Romalde Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Juan L. Barja Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Keywords: Phaeobacter, probiotics, mollusc larval pathogens, antibacterial activity Abstract A total of 523 bacterial strains were isolated during a 4-year period from mollusc hatcheries (flat oyster and clams) in Galicia (NW Spain). All of the strains were tested for their antibacterial activity against three larval pathogens (Vibrio anguillarum USC-72, V. neptunius PP-145.98, and Vibrio sp. PP-203). Of the isolates, 52 inhibited at least one of the target strains, and 11 inhibited all of them. The main source of active strains was oyster larvae, followed by water, tank surfaces, spat, and broodstock. Four similar strains, belonging to the genus Phaeobacter, showed the strongest activity. Strain PP-154, selected as representative of this group, displayed a wide spectrum of inhibitory activity against aquaculture pathogens, especially against members of the genus Vibrio, which is responsible for the most larval deaths. The inhibitory ability of such strain on solid medium was confirmed in seawater experiments, and the optimal conditions for antibacterial activity were established. These strains are promising probiotics for aquaculture facilities. Their potential benefit is based on the capacity to control the proliferation of a variety of aquaculture bacterial pathogens in mollusc larval cultures. [Int Microbiol 2009; 12(2):107-114] Author Biographies Susana Prado, Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Jaime Montes, Center of Marine Research, Ministry of Fisheries and Marine Affairs, Autonomous Government of Galicia, Vilanova de Arousa, Spain Center of Marine Research, Ministry of Fisheries and Marine Affairs, Autonomous Government of Galicia, Vilanova de Arousa, Spain Jesús L. Romalde, Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Juan L. Barja, Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Department of Microbiology and Parasitology, Faculty of Biology and Institute of Aquaculture, University of Santiago de Compostela, Santiago de Compostela, Spain Downloads PDF Published 2010-01-13 Issue Vol. 12 No. 2 (2009) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
