Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture Authors Inmaculada Moreno GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Nunzia Tutrone GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Rafael Sentandreu GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Eulogio Valentín GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Abstract Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared from a S. cerevisiae Rds2 mutant strain. Analyses of the sensitivity of this rds2Δ mutant to different drugs and to osmotic stress showed that Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity. [Int Microbiol 2008; 11(1): 57-63] Author Biographies Inmaculada Moreno, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Nunzia Tutrone, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Rafael Sentandreu, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Eulogio Valentín, GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain GMCA Research Group, Department of Microbiology and Ecology, Faculty of Pharmacy, University of Valencia, Spain Downloads PDF Published 2010-01-19 Issue Vol. 11 No. 1 (2008) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
