Influence of a Streptomyces lividans SecG functional analogue on protein secretion Authors Carmen Palomino National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain Rafael P. Mellado National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain Keywords: Streptomyces lividans, translocase, SecG, protein secretion Abstract The membrane protein complex translocase mediates the translocation of bacterial proteins. In this complex, the SecY, SecE, and SecG proteins constitute an integral membrane domain. Sequence comparison revealed a potential secGlike gene in the gram-positive soil bacterium Streptomyces lividans. Chromosomal deletion of this gene resulted in a sporulation defect and an overall deficiency in secretion. The SecG-depleted strain was able to overproduce and secrete α-amylase, but the appearance of the oversynthesized protein outside the cell was delayed compared to the protein produced by the wildtype strain. SecG deficiency was found to result in more pronounced effects in S. lividans than in Bacillus subtilis or Escherichia coli. [Int Microbiol 2008; 11(1):25-31] Author Biographies Carmen Palomino, National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain Rafael P. Mellado, National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain National Center for Biotechnology, CSIC, Autonomous University of Madrid, Cantoblanco, Spain Downloads PDF Published 2010-01-19 Issue Vol. 11 No. 1 (2008) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
