Analysis of s-triazine-degrading microbial communities in soils using most-probable-number enumeration and tetrazolium-salt detection Authors M. Alejandro Dinamarca Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa María, Valparaíso, Chile Francisco Cereceda-Balic Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa María, Valparaíso, Chile Ximena Fadic Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa María, Valparaíso, Chile Michael Seeger Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa María, Valparaíso, Chile Keywords: Pseudomonas sp. ADP, s-triazine, simazine, atrazine, tetrazolium salt, herbicides, biodegradation Abstract A simple and sensitive method for the detection and enumeration of microbial s-triazine-degrading microorganisms in soil was designed. The procedure is based on the ability of some microbes to use s-triazines, such as simazine, atrazine, and cyanuric acid, as sole nitrogen source. It employs the respiration indicator 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) to detect metabolic activity and the most-probable-number (MPN) enumeration in microtiter plates. The method was used to identify simazine- and cyanuric acid-degrading activities in agricultural soils treated with the herbicide simazine. The MPN-TTC method showed that the number of simazine- and cyanuric acid-degrading microorganisms increased four weeks after the herbicide simazine had been applied. [Int Microbiol 2007; 10(3):209-215] Author Biographies M. Alejandro Dinamarca, Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa María, Valparaíso, Chile Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa MarÃa, ValparaÃso, Chile Laboratory of Microbial Biotechnology, Faculty of Pharmacy, University of ValparaÃso, ValparaÃso, Chile Francisco Cereceda-Balic, Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa María, Valparaíso, Chile Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa MarÃa, ValparaÃso, Chile Ximena Fadic, Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa María, Valparaíso, Chile Laboratory of Environmental Chemistry, Department of Chemistry, Technical University Federico Santa MarÃa, ValparaÃso, Chile Michael Seeger, Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa María, Valparaíso, Chile Laboratory of Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Millennium Nucleus of Microbial Ecology and Environmental Microbiology and Biotechnology, Technical University Federico Santa MarÃa, ValparaÃso, Chile Downloads PDF Published 2010-01-22 Issue Vol. 10 No. 3 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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