Co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish Authors Esther García-Rosado Department of Microbiology, University of Málaga. Málaga, Spain Irene Cano Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain Beatriz Martín-Antonio Department of Microbiology, University of Málaga. Málaga, Spain Alejandro Labella Department of Microbiology, University of Málaga. Málaga, Spain Manuel Manchado IFAPA El Toruño Center, Junta de Andalucía, El Puerto de Santa María, Cádiz, Spain M. Carmen Alonso Department of Microbiology, University of Málaga. Málaga, Spain Dolores Castro Department of Microbiology, University of Málaga. Málaga, Spain Juan J. Borrego Department of Microbiology, University of Málaga. Málaga, Spain Keywords: Vibrio spp., Photobacterium damselae subsp. damselae, Virus, VNNV, VHSV, sparid fish, co-occurrence of pathogens Abstract Several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as common seabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. This study describes the isolation and characterization of the potential causative agents, either bacteria or viruses, of these outbreaks. The isolated bacterial strains were characterized according to traditional taxonomical analyses and sequencing of a 16S rDNA fragment. Most bacteria were identified as Vibrio spp. and Photobacterium damselae subsp. damselae. The development of cytopathic effects (CPE) on different fish cell lines, the application of specific nested-PCR tests for infectious pancreatic necrosis virus (IPNV), viral nervous necrosis virus (VNNV) and viral hemorrhagic septicemia virus (VHSV), and subsequent sequence analyses were used for virus detection and identification. VNNV, related to the striped jack neural necrosis virus (SJNNV) genotype, and VHSV, related to the genotype Ia, were the only viruses detected. VNNV was isolated from the three fish species under study in five different outbreaks, whereas VHSV was isolated from common seabream and white seabream during two of these outbreaks. IPNV was not detected in any case. [Int Microbiol 2007; 10(3):193-199] Author Biographies Esther García-Rosado, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Irene Cano, Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain Department of Microbiology, University of Málaga. Málaga, Spain Institute for Marine Sciences, CSIC, Puerto Real, Cádiz, Spain Beatriz Martín-Antonio, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Alejandro Labella, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Manuel Manchado, IFAPA El Toruño Center, Junta de Andalucía, El Puerto de Santa María, Cádiz, Spain IFAPA El Toruño Center, Junta de AndalucÃa, El Puerto de Santa MarÃa, Cádiz, Spain M. Carmen Alonso, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Dolores Castro, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Juan J. Borrego, Department of Microbiology, University of Málaga. Málaga, Spain Department of Microbiology, University of Málaga. Málaga, Spain Downloads PDF Published 2010-01-22 Issue Vol. 10 No. 3 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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