Dominant cultivable Lactobacillus species from the feces of healthy adults in northern Spain Authors Susana Delgado Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Adolfo Suárez Gastrointestinal Service, Hospital of Cabueñes, Gijón, Spain Baltasar Mayo Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Keywords: Lactobacillus spp., L. paracasei, L. gasseri, L. delbrueckii, L. plantarum, gastrointestinal tract, intestinal microbiology, human microbiota, probiotics Abstract The aim of this study was to identify numerically dominant cultivable lactobacilli species in the feces of healthy adults. Ten individuals from Asturias, northern Spain, were chosen. Bacterial colonies grown under anoxic conditions on MRS with cysteine were microscopically examined for lactobacilli. Isolates were subsequently grouped based on the analysis of their carbohydrate fermentation profiles and then identified by partial amplification, sequencing, and comparison of their 16S rRNA gene sequences. Lactobacilli varied from undetectable levels in three subjects (< 105 CFU/g feces) to around 109 CFU/g feces. Among the 71 isolates obtained from seven individuals, 12 Lactobacillus species were identified. High interindividual variation was observed in terms of total numbers, number of species, and dominant species. Lactobacillus paracasei was found in four of the seven individuals; L. gasseri, L. delbrueckii, and L. plantarum in three. Phenotyping showed that only one strain per species was in the majority in each individual. [Int Microbiol 2007; 10(2):141-145] Author Biographies Susana Delgado, Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Adolfo Suárez, Gastrointestinal Service, Hospital of Cabueñes, Gijón, Spain Gastrointestinal Service, Hospital of Cabueñes, Gijón, Spain Baltasar Mayo, Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Institute of Dairy Products of Asturias, CSIC, Villaviciosa, Spain Downloads PDF Published 2010-01-26 Issue Vol. 10 No. 2 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
