Molecular fingerprinting of Campylobacter and Arcobacter isolated from chicken and water

Authors

  • Ana González Department of Biotechnology, Polytechnic University of Valencia, Spain
  • María A. Ferrús Department of Biotechnology, Polytechnic University of Valencia, Spain
  • Rosa González Department of Biotechnology, Polytechnic University of Valencia, Spain
  • Javier Hernández Department of Biotechnology, Polytechnic University of Valencia, Spain

Keywords:

Campylobacter, Arcobacter, single-enzyme amplified fragment length polymorphism (s-AFLP), pulsed-field gel electrophoresis (PFGE)

Abstract

The potential of a fingerprinting method based on the single-enzyme amplified fragment length polymorphism (s-AFLP) technique was evaluated for its efficacy in detecting foodborne Campylobacter and Arcobacter species. Campylobacter and Arcobacter isolates from chicken and water samples were subjected to s-AFLP and pulsed-field gel electrophoresis (PFGE) profiling. Molecular typing revealed a high degree of heterogeneity. AFLP was found to be appropriate for differentiating minimal genomic variations, which makes this technique a valuable tool for the identification of isolates. PFGE was effective in showing epidemiological relationships among closely related isolates. Either technique allowed the discrimination of A. butzleri from A. cryaerophilus and A. skirrowii. When used together, s-AFLP and PFGE can be applied to determine taxonomic and epidemiological relationships among campylobacteria. [Int Microbiol 2007; 10(2):85-90]

Author Biographies

Ana González, Department of Biotechnology, Polytechnic University of Valencia, Spain

Department of Biotechnology, Polytechnic University of Valencia, Spain

María A. Ferrús, Department of Biotechnology, Polytechnic University of Valencia, Spain

Department of Biotechnology, Polytechnic University of Valencia, Spain

Rosa González, Department of Biotechnology, Polytechnic University of Valencia, Spain

Department of Biotechnology, Polytechnic University of Valencia, Spain

Javier Hernández, Department of Biotechnology, Polytechnic University of Valencia, Spain

Department of Biotechnology, Polytechnic University of Valencia, Spain

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Published

2010-01-26

Issue

Section

Research Articles