Molecular fingerprinting of Campylobacter and Arcobacter isolated from chicken and water Authors Ana González Department of Biotechnology, Polytechnic University of Valencia, Spain María A. Ferrús Department of Biotechnology, Polytechnic University of Valencia, Spain Rosa González Department of Biotechnology, Polytechnic University of Valencia, Spain Javier Hernández Department of Biotechnology, Polytechnic University of Valencia, Spain Keywords: Campylobacter, Arcobacter, single-enzyme amplified fragment length polymorphism (s-AFLP), pulsed-field gel electrophoresis (PFGE) Abstract The potential of a fingerprinting method based on the single-enzyme amplified fragment length polymorphism (s-AFLP) technique was evaluated for its efficacy in detecting foodborne Campylobacter and Arcobacter species. Campylobacter and Arcobacter isolates from chicken and water samples were subjected to s-AFLP and pulsed-field gel electrophoresis (PFGE) profiling. Molecular typing revealed a high degree of heterogeneity. AFLP was found to be appropriate for differentiating minimal genomic variations, which makes this technique a valuable tool for the identification of isolates. PFGE was effective in showing epidemiological relationships among closely related isolates. Either technique allowed the discrimination of A. butzleri from A. cryaerophilus and A. skirrowii. When used together, s-AFLP and PFGE can be applied to determine taxonomic and epidemiological relationships among campylobacteria. [Int Microbiol 2007; 10(2):85-90] Author Biographies Ana González, Department of Biotechnology, Polytechnic University of Valencia, Spain Department of Biotechnology, Polytechnic University of Valencia, Spain María A. Ferrús, Department of Biotechnology, Polytechnic University of Valencia, Spain Department of Biotechnology, Polytechnic University of Valencia, Spain Rosa González, Department of Biotechnology, Polytechnic University of Valencia, Spain Department of Biotechnology, Polytechnic University of Valencia, Spain Javier Hernández, Department of Biotechnology, Polytechnic University of Valencia, Spain Department of Biotechnology, Polytechnic University of Valencia, Spain Downloads PDF Published 2010-01-26 Issue Vol. 10 No. 2 (2007) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
