Phylogenetic position of parabasalid symbionts from the termite Calotermes flavicollis based on small subunit rRNA sequences Authors Delphine Gerbod Laboratoire de Biologie Comparée des Protistes, UPRESA CNRS 6023, Aubière, France Virginia P. Edgcomb Center for Molecular Evolution, Marine Biological Laboratory, Woods Hole, USA Christophe Noël Laboratoire de Biologie Comparée des Protistes, UPRESA CNRS 6023, Aubière, France; and Institut Pasteur, INSERM U167, Lille, France Pilar Delgado-Viscogliosi Laboratoire d’Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France; and Institut Pasteur, Eaux et Environnement, Lille, France Eric Viscogliosi Laboratoire de Biologie Comparée des Protistes, UPRESA CNRS 6023, Aubière, France; and Institut Pasteur, INSERM U167, Lille, France Keywords: parabasalid protists, termites, small subunit rRNA, phylogeny, molecular evolution Abstract Small subunit rDNA genes were amplified by polymerase chain reaction using specific primers from mixed-population DNA obtained from the whole hindgut of the termite Calotermes flavicollis. Comparative sequence analysis of the clones revealed two kinds of sequences that were both from parabasalid symbionts. In a molecular tree inferred by distance, parsimony and likelihood methods, and including 27 parabasalid sequences retrieved from the data bases, the sequences of the group II (clones Cf5 and Cf6) were closely related to the Devescovinidae/Calonymphidae species and thus were assigned to the Devescovinidae Foaina. The sequence of the group I (clone Cf1) emerged within the Trichomonadinae and strongly clustered with Tetratrichomonas gallinarum. On the basis of morphological data, the Monocercomonadidae Hexamastix termitis might be the most likely origin of this sequence. Downloads PDF Published 2010-03-14 Issue Vol. 3 No. 3 (2000) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
