Changes in protein synthesis and acid tolerance in Clostridium perfringens type A in response to acid shock

Authors

  • Licet Villarreal Faculty of Biological Sciences, Autonomous University of Nuevo León, San Nicolás, N.L., México
  • Norma L. Heredia Faculty of Biological Sciences, Autonomous University of Nuevo León, San Nicolás, N.L., México
  • Santos García Faculty of Biological Sciences, Autonomous University of Nuevo León, San Nicolás, N.L., México

Abstract

The induction of acid-shock proteins and the degree of acid resistance conferred on Clostridium perfringens by acid shock, and the kinetics of this resistance were determined. A sublethal acid shock at pH 4.5 for 20 min increased the acid tolerance of cells at least fifteenfold. The acquired tolerance was maintained for 3 h after acid treatment. The response of the microorganism to acid shock was also examined by analysis of pulse-labeled proteins. Five acid-shock proteins (molecular weights 120, 84, 58, 45 and 17 kDa) were identified by polyacrylamide gel electrophoresis.

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Published

2010-03-15

Issue

Vol. 3 No. 2 (2000)

Section

Research Articles

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