Characterization of Bacillus strains of marine origin Authors Elena P. Ivanova Pacific Institute of Bio-organic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia; an Institute of Marine Biology, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Mikhail V. Vysotskii Institute of Marine Biology, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Vasilii I. Svetashev Institute of Marine Biology, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Olga I. Nedashkovskaya Pacific Institute of Bio-organic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Natalia M. Gorshkova Pacific Institute of Bio-organic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Valery V. Mikhailov Pacific Institute of Bio-organic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia Noboru Yumoto Osaka National Research Institute, AIST, Ikeda, Osaka, Japan Yasushi Shigeri Osaka National Research Institute, AIST, Ikeda, Osaka, Japan Takahisa Taguchi Osaka National Research Institute, AIST, Ikeda, Osaka, Japan Susumu Yoshikawa Osaka National Research Institute, AIST, Ikeda, Osaka, Japan Keywords: Bacillus spp., phenotypic characterization, fatty acid analysis, marine microbiology Abstract A total of twenty aerobic endospore-forming bacilli, isolated from marine invertebrates and sea water of different areas of the Pacific Ocean, were taxonomically characterized. Most of the bacilli (11 strains) of marine origin belonged to the species Bacillus subtilis, according to their phenotypic characteristics, antibiotic susceptibility profiles, and fatty acids patterns. A group of four alkaliphilic strains formed a separate cluster that was tentatively classified as B. horti. One isolate, KMM 1717, associated with a sponge from the Coral Sea was identified as B. pumilus. Two strains, Bacillus KMM 1916 and KMM 1918, showed antibiotic sensitivity profiles similar to B. licheniformis, but they had a distinct fatty acid composition and peculiar phenotypic traits. The taxonomic affiliation of KMM 1810 and KMM 1763 remained unclear since their fatty acid composition and antibiotic sensitivity patterns were not resembled with none of these obtained for Bacillus strains. Downloads PDF Published 2010-03-16 Issue Vol. 2 No. 4 (1999) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.
