A rapid procedure for the isolation of plasmid DNA from environmental bacteria

Authors

  • María A. Ferrús Department of Biotechnology, Polytechnic University of Valencia, Spain
  • José L. Alonso Hydrology and Environmental Institute, Polytechnic University of Valencia, Spain
  • Inmaculada Amorós Hydrology and Environmental Institute, Polytechnic University of Valencia, Spain
  • Manuel Hernández Department of Biotechnology, Polytechnic University of Valencia, Spain
  • Javier Hernández Department of Biotechnology, Polytechnic University of Valencia, Spain

Keywords:

Pseudomonas aeruginosa, Escherichia coli, Salmonella, plasmidscreening procedure, plasmid extraction

Abstract

The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environmental Salmonella strains, although it was not suitable for Pseudomonas aeruginosa and enterococci strains. The INSTA-MINI-PREP method is a new alternative procedure to screen plasmid contents of Salmonella and E. coli strains rapidly and easily.

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Published

2010-03-17

Issue

Vol. 2 No. 2 (1999)

Section

Research Articles

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