Non-conventional yeasts as hosts for heterologous protein production Authors Ángel Domínguez Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Encarnación Fermiñán Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Manuel Sánchez Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Francisco J. González Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Flor María Pérez-Campo Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Susana García Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Ana B. Herrero Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Avelino San Vicente Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Juan Cabello Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Marciano Prado Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Francisco J. Iglesias Departamento de Física Aplicada, Universidad de Salamanca, Spain Altino Choupina Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain; and Departamento de Química Física, Universidad de Salamanca, Spain Francisco J. Burguillo Departamento de Química Física, Universidad de Salamanca, Spain Luis Fernández-Lago Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain María Carmen López Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain Keywords: Kluyveromyces lactis, Yarrowia lipolytica, Pichia pastoris, Hansenula polymorpha, heterologous protein Abstract Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers’ yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae. Downloads PDF Published 2010-03-18 Issue Vol. 1 No. 1 (1998) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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