Phylogenetic diversity of sediment bacteria from the deep Northeastern Pacific Ocean: a comparison with the deep Eastern Mediterranean Sea Authors Ioanna Kouridaki Department of Chemistry, University of Crete, Heraklion, Crete, Greece. Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece. Paraskevi N. Polymenakou Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Anastasios Tselepides Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece. Department of Maritime Studies, University of Piraeus, Piraeus, Greece Manolis Mandalakis Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Kenneth L. Smith, Jr Monterey Bay Aquarium Research Institute, Moss Landing, California, USA Abstract The variability of bacterial community composition and diversity was studied by comparative analysis of five 16S rRNA gene clone libraries from deep-sea sediments (water column depth: 4000 m) of the Northeastern Pacific Ocean and Eastern Mediterranean Sea. This is the first comparison of the bacterial communities living in these deep-sea ecosystems. The estimated chlorophyll a, organic carbon, and C/N ratio provided evidence of significant differences in the trophic state of the sediments between the Northeastern Pacific Ocean and the much warmer Eastern Mediterranean Sea. A diverse range of 16S rRNA gene phylotypes was found in the sediments of both regions. These were represented by 11 different taxonomic groups, with Gammaproteobacteria predominating in the Northeastern Pacific Ocean sediments and Acidobacteria in the Eastern Mediterranean microbial community. In addition, several 16S rRNA gene phylotypes only distantly related to any of the previously identified sequences (non-affiliated rRNA genes) represented a significant fraction of the total sequences. The potential diversity at the two sites differs but remains largely unexplored and remains of continuing scientific interest. [Int Microbiol 2010; 13(3):143-150] Author Biographies Ioanna Kouridaki, Department of Chemistry, University of Crete, Heraklion, Crete, Greece. Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece. Department of Chemistry, University of Crete, Heraklion, Crete, Greece.Institute of Marine Biology and Genetics,Hellenic Centre for Marine Research, Heraklion, Crete, Greece. Paraskevi N. Polymenakou, Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Anastasios Tselepides, Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece. Department of Maritime Studies, University of Piraeus, Piraeus, Greece Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, GreeceDepartment of Maritime Studies, University of Piraeus, Piraeus, Greece Manolis Mandalakis, Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, Heraklion, Crete, Greece Kenneth L. Smith, Jr, Monterey Bay Aquarium Research Institute, Moss Landing, California, USA Monterey Bay Aquarium Research Institute, Moss Landing, California, USA Downloads PDF Published 2010-09-28 Issue Vol. 13 No. 3 (2010) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. 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