Coordination between replication, segregation and cell division in multi-chromosomal bacteria: lessons from Vibrio cholerae Authors Elena Espinosa François-Xavier Barre Elisa Galli Keywords: Bacteria display a highly flexible cell cycle in which cell division can be temporally disconnected from the replication/ segregation cycle of their genome. The accuracy of genetic transmission is enforced by restricting the assembly of the cell division Abstract Bacteria display a highly flexible cell cycle in which cell division can be temporally disconnected from the replication/segregation cycle of their genome. The accuracy of genetic transmission is enforced by restricting the assembly of thecell division apparatus to the low DNA-density zones that develop between the regularly spaced nucleoids originating from theconcurrent replication and segregation of genomic DNA. In most bacteria, the process is simplified because the genome is encodedon a single chromosome. This is notably the case in Escherichia coli, the most well studied bacterial model organism. However,~10% of bacteria have domesticated horizontally acquired mega-plasmids into extra-numerous chromosomes. Most of our currentknowledge on the cell cycle regulation of multi-chromosomal species derives from the study of replication, segregation and celldivision in Vibrio cholerae, the agent of the deadly epidemic human diarrheal disease cholera. A nicety of this model is that it isclosely related to E. coli in the phylogenetic tree of bacteria. Here, we review recent findings on the V. cholerae cell cycle in thecontext of what was previously known on the E. coli cell cycle. Author Biographies Elena Espinosa Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris Sud, 1 avenue dela Terrasse, 91198 Gif sur Yvette, France François-Xavier Barre Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris Sud, 1 avenue dela Terrasse, 91198 Gif sur Yvette, France Elisa Galli Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris Sud, 1 avenue dela Terrasse, 91198 Gif sur Yvette, France Downloads PDF Published 2018-02-27 Issue Vol. 20 No. 3 (2017) Section Research Reviews License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.