Haloalkalitolerant Actinobacteria with capacity for anthracene degradation isolated from soils close to areas with oil activity in the State of Veracruz, Mexico

Authors

  • Reyna del C. Lara-Severino Faculty of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico.
  • Miguel A. Camacho-López Faculty of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico.
  • Edgar Casanova-González National Coordination of the de Preservation of Cultural Heritage, National Institute of Anthropology and History, Mexico DF, Mexico.
  • Leobardo M. Gómez-Oliván Faculty of Chemistry, Autonomous University of the State of Mexico, Toluca, Mexico.
  • Ángel H. Sandoval-Trujillo Department of Biological Systems, Autonomous University Metropolitana-Xochimilco, Mexico DF, Mexico
  • Keila Isaac-Olivé Faculty of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico.
  • Ninfa Ramírez-Durán Faculty of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico.

Keywords:

Kocuria, Microbacterium, haloalkalitolerant Actinobacteria, anthracene degradation, State of Veracruz, Mexico

Abstract

The use of native strains of microorganisms from soils is an excellent option for bioremediation. To our knowledge, until now there has been no other group working on the isolation of Actinobacteria from contaminated soils in Mexico. In this study, samples of soils close to areas with oil activity in the State of Veracruz, Mexico, were inoculated for the isolation of Actinobacteria. The strains isolated were characterized morphologically, and the concentrations of NaCl and pH were determined for optimal growth. Strain selection was performed by the detection of a phylogenetic marker for Actinobacteria located at the 23S rRNA gene, followed by species identification by sequencing the 16S rRNA gene. Several haloalkalitolerant Actinobacteria were isolated and identified as: Kocuria rosea, K. palustris, Microbacterium testaceum, Nocardia farcinica and Cellulomonas denverensis. Except for C. denverensis, the biomass of all strains increased in the presence of anthracene. The strains capacity to metabolize anthracene (at 48 h), determined by fluorescence emission, was in the range of 46–54%. During this time, dihydroxy aromatic compounds formed, characterized by attenuated total reflectance Fourier transform infrared spectroscopy bands of 1205 cm–1 and 1217 cm–1. Those Actinobacteria are potentially useful for the bioremediation of saline and alkaline environments contaminated with polycyclic aromatic hydrocarbon compounds. [Int Microbiol 2016; 19(1):15-26]

Keywords: Kocuria · Microbacterium · haloalkalitolerant Actinobacteria · anthracene degradation · State of Veracruz, Mexico

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Issue

Vol. 19 No. 1 (2016)

Section

Research Articles

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