Properties of Lactobacillus reuteri chitosan-calcium-alginate encapsulation under simulated gastrointestinal conditions


  • Hui-Ying Huang Department of Nutrition, China Medical University, Taichung
  • Yi-Ju Tang Department of Nutrition, China Medical University, Taichung
  • V. An-Erl King Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan
  • Jen-Wei Chou Division of Gastroenterology and Hepatology, Department of Internal Medicine, China Medical University Hospital, College of Medicine, China Medical University, Taichung.
  • Jen-Horng Tsen Department of Nutrition, China Medical University, Taichung.


Lactobacillus reuteri, Listeria monocytogenes, chitosan–calcium-alginate encapsulation, probiotic properties, simulated gastrointestinal conditions


The protective effects of encapsulation on the survival of Lactobacillus reuteri and the retention of the bacterium’s probiotic properties under simulated gastrointestinal conditions were investigated. Viable counts and the remaining probiotic properties of calcium (Ca)-alginate encapsulated (A group), chitosan-Ca-alginate encapsulated (CA group), and unencapsulated, free L. reuteri (F group) were determined. Encapsulation improved the survival of L. reuteri subjected to simulated gastrointestinal conditions, with the greatest protective effect achieved in the CA group. The degree of cell membrane injury increased with increasing bile salt concentrations at constant pH, but the extent of injury was less in the encapsulated than in the free cells. Adherence rates were, in descending order: CA (0.524%) > A (0.360%) > F (0.275%). Lactobacillus reuteri cells retained their antagonistic activity toward Listeria monocytogenes even after incubation of the lactobacilli under simulated gastrointestinal conditions. Displacement of the pathogen by cells released from either of the encapsulation matrices was higher than that by free cells. The safety of L. reuteri was demonstrated in an in vitro invasion assay. [Int Microbiol 2015; 18(1):61-69]

Keywords: Lactobacillus reuteri · Listeria monocytogenes · chitosan–calcium-alginate encapsulation · probiotic properties · simulated gastrointestinal conditions

Author Biography

Hui-Ying Huang, Department of Nutrition, China Medical University, Taichung






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