Metabolomic charactetization of yeast cells after dehydration stress

Authors

  • Gema López-Martínez Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona.
  • Anna Borrull Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona.
  • Montse Poblet Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona.
  • Nicolas Rozès Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona.
  • Ricardo Cordero-Otero Department of Biochemistry and Biotechnology, University Rovira Virgili, Tarragona.

Keywords:

Saccaromyces mikatae, Saccharomyces cerevisiae, Naumovia castellii, viability, dehydration stress, metabolite extraction, wild yeast

Abstract

In this study, we analyzed the metabolite features of the yeasts Saccharomyces cerevisiae, Naumovia castellii, and Saccharomyces mikatae. The three species are closely related genetically but differ in their tolerance of desiccation stress. Specifi cally, we determined whether certain metabolites correlated with cell viability after stress imposition. The metabolomic profi les of these strains were compared before cell desiccation and after cell rehydration. In S. mikatae, the presence of lysine or glutamine during rehydration led to a 20% increase in survival whereas during dehydration the levels of both amino acids in this yeast were drastically reduced. [Int Microbiol 2014; 17(3):131-139]  

Keywords: Saccaromyces mikatae · Saccharomyces cerevisiae · Naumovia castellii · viability · dehydration stress · metabolite extraction · wild yeast

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Issue

Vol. 17 No. 3 (2014)

Section

Research Articles

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