Acetosyringone induces the accumulation of a set of RNAs in the Arbuscular Mycorrhiza fungus Glomus intraradices Authors Estela Flores-Gomez Departamento de Biotecnología y Bioingeniería, CINVESTAV Lidia Gomez-Silva Departamento de Biotecnología y Bioingeniería, CINVESTAV R. Ruiz-Medrano Departamento de Biotecnología y Bioingeniería, CINVESTAV B. Xoconostle-Cázares Departamento de Biotecnología y Bioingeniería, CINVESTAV Keywords: Arbuscular mycorrhiza, Acetosyringone, Differential Expression, Macroarrays Abstract Plant root exudates contain a range of low molecular weight metabolites, which are believed to trigger the structural and physiological changes associated with the progression and establishment of the mycorrhizal symbiosis. Glomus intraradices spores were incubated with acetosyringone (AS), and an overall increase in the hyphal respiration was observed, indicating a physiological response triggered by this plant regulator. A G. intraradices cDNA library was then screened with a total cDNA probe obtained from the AS-treated spores and mycelium. The cDNAs from different functional categories were identified as induced in G. intraradices when exposed to AS, such as protein synthesis, membrane transport, signal transduction, general metabolism, without assigned function, and no identity. Interestingly, a cDNA coding for a fragment of a histidine kinase was also induced by AS, suggesting a two-component mediated response. We also demonstrated the differential accumulation of a cruciform DNA-binding protein mRNA, termed as GiBP1. Time-course experiments demonstrated its rapid accumulation within 2h of induction with AS. These results indicate the presence of a set of fungal genes that are induced by the presence of the inducer. These findings are discussed in terms of the possible molecular events that follow the exchange of signals between the mycorrhizal symbionts. Author Biographies Estela Flores-Gomez, Departamento de Biotecnología y Bioingeniería, CINVESTAV Departamento de Biotecnología y Bioingeniería, CINVESTAV Lidia Gomez-Silva, Departamento de Biotecnología y Bioingeniería, CINVESTAV Departamento de Biotecnología y Bioingeniería, CINVESTAV R. Ruiz-Medrano, Departamento de Biotecnología y Bioingeniería, CINVESTAV Departamento de Biotecnología y Bioingeniería, CINVESTAV B. Xoconostle-Cázares, Departamento de Biotecnología y Bioingeniería, CINVESTAV Departamento de Biotecnología y Bioingeniería, CINVESTAV Downloads PDF Published 2010-09-21 Issue Vol. 11 No. 4 (2008) Section Research Articles License Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <http://www.im.microbios.org>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.